Acta Pharm. 68 (2018) 129-144
Original research paper
Hydroxyapatite-ciprofloxacin delivery system: Synthesis, characterisation and antibacterial activity
MARIA-VIORICA CIOCILTEU, ANDREEA GABRIELA MOCANU, ADRIANA MOCANU, CATALIN DUCU, OANA ELENA NICOLAESCU, VALENTIN COSTEL MANDA, ADINA TURCU-STIOLICA, CLAUDIU NICOLICESCU, RAZVAN MELINTE, MARIA BALASOIU, OCTAVIAN CROITORU and JOHNY NEAMTU
1 University of Medicine and Pharmacy of Craiova, Faculty of Pharmacy, Craiova (Dolj), Romania
2 CS I, Global Research S.R.L., Pitešti
3 University of Craiova, Faculty of Mechanics, Department of IMST, 220037, Drobeta Turnu Severin (Mehedinti), Romania
4 University of Medicine and Pharmacy of Craiova, Faculty of Medicine, Craiova (Dolj), Romania
Accepted December 9, 2017
Published online February 6, 2018
The main objective of this study was to synthesize hydroxyapatite-ciprofloxacin composites using a chemical precipitation method and to evaluate the properties and in vitro release profile of the drug from the hydroxyapatite-ciprofloxacin composites. Composite characterization was achieved by FT-IR, XRD and DLS. Ciprofloxacin determination was accomplished by HPLC, resulting in good incorporation efficiency of the drug (18.13 %). The in vitro release study (Higuchi model C = K t1/2 and Ritger-Peppas model, C = K t0.6) showed a diffusion-controlled mechanism. The antibacterial activity showed that the bacterial growth inhibition zones were approximately equal for the synthesis composites and for the mechanical mixture on the Staphylococcus aureus germ.
The use of hydroxyapatite, which is a biocompatible, bioactive and osteoconductive material, with ciprofloxacin, which has good antibacterial activity in this composite, makes it suitable for the development of bone grafts. Furthermore, the synthesis process allows a slow local release of the drug.
Keywords: hydroxiapatite-ciprofloxacin composites, wet precipitation synthesis, in vitro release profile, antibacterial activity