Acta Pharm. 67 (2017) 169-186

 

full paper

Original research paper

 

Quantitative analysis of povidone-iodine thin films by X-ray photoelectron spectroscopy and their physicochemical properties

ANONGTIP SA, SOMCHAI SAWATDEE, NARUBODEE PHADOONGSOMBUT, WILAIPORN BUATONG, TITPAWAN NAKPENG, RUTTHAPOL SRITHARADOL and TEERAPOL SRICHANA

teerapol.s@psu.ac.th, somchai.sa@wu.ac.th, somchai086@hotmail.com

1 Department of Pharmaceutical Technology, Faculty of Pharmaceutical Sciences, Prince of Songkla University, Hat Yai, Songkhla 90112, Thailand

2 Drug and Cosmetic Research and Development Unit, School of Pharmacy, Walailak University, Thasala, Nakhon Si Thammarat 80161, Thailand

3 Department of Pharmaceutical Chemistry, Faculty of Pharmaceutical Sciences, Prince of Songkla University, Hat Yai, Songkhla 90112, Thailand

4 Nanotec-PSU Excellence Center on Drug Delivery System, Faculty of Pharmaceutical Sciences, Prince of Songkla University, Hat Yai, Songkhla 90112, Thailand

Accepted December 19, 2016

Published online February 20, 2016

 

In this study, povidone-iodine (PVP-I) has been formulated as a topical spray to produce a thin film for the controlled release of I2. By means of experimental design, 27 formulations containing glycerol, ethanol, PEG 400, copovidone and HFA 134a as a propellant were prepared. The pH values of all formulations were in the range of 67. The viscosity was within the range of 11.985.9 mPas. The surface tensions were 20.3 to 24.6 mN m1 and the contact angles were between 19.3 and 38.7. The assays for the iodine contents were within acceptable range (80120 %). X-ray photoelectron spectroscopy analysis revealed the ionized form of iodine was much higher than the unionized form. The MIC and MBC values of the PVP-I sprays against Staphylococcus aureus, S. epidermidis and Pseudomonas aeruginosa were higher than that of commercial PVP-I solution. The cytotoxicity study confirmed that the PVP-I spray had lower toxic effects on keratinocytes and fibroblasts compared to the commercial PVP-I solution. The formulation containing 59 % ethanol, 18 % copovidone and 12 % PEG 400 showed good antibacterial activity.

 

Keywords: povidone-iodine, topical spray, bioactivity, cytotoxicity, X-ray photoelectron spectroscopy