Acta Pharm. 66 (2016) 233-244

 

full paper

Original research paper

 

Evaluation of the topical spray containing Centella asiatica extract and efficacy on excision wounds in rats

SOMCHAI SAWATDEE, KANUENGNIT CHOOCHUAY, WIROT CHANTHORN and TEERAPOL SRICHANA

somchai086@hotmail.com, somchai.sa@wu.ac.th

1 School of Pharmacy, Walailak University, Thasala District, Nakhon Si Thammarat, 80161, Thailand

2 Drug and Cosmetic Research and Development Unit, School of Pharmacy, Walailak University, Thasala District, Nakhon Si Thammarat, 80161, Thailand

3 Faculty of Public Health, Naresuan University, Tha Pho, Muang, Phitsanulok, 65000, Thailand

4 Department of Pharmaceutical Technology and Nanotec-PSU Excellence Center on Drug Delivery System, Faculty of Pharmaceutical Sciences, Prince of Songkla University, Hat Yai, Songkhla 90112, Thailand

Accepted January 20, 2016

Published online May 16, 2016

 

Centella asiatica was extracted by methanol. The assay content of triterpenes in the extract was 0.12 % asiatic acid, 0.54 % madecassic acid, 0.25 % asiaticoside and 1.02 % madecassoside. The extract was complexed with hydroxypropyl-b-cyclodextrin (HP-b-CD) and formulated with Eudragit E100, glycerol, PEG 400, copovidone, ethanol and purified water. A clear yellowish solution (F1-F8) was obtained. The formulations had a pH of 5.56.0 with viscosity in the range of 2060 mPa s, surface tension 20.324.6 mN m1 and contact angle less than 20. The amount of PEG 400 and copovidone affected the film and spreadability. The content of triterpenes in the spray formulation was close to 100 % compared to triterpenes in the extract. The skin irritation study indicated that the formulation was non-irritating in a rat model. An in vivo excision wound healing model showed that wound excision was completely healed after 14 days.

 

Keywords: Centella asiatica extract, hydroxypropyl-b-cyclodextrin, wound healing, topical spray