Acta Pharm. 68 (2018) 373-380

 

full paper

Short communication

 

In vivo investigation of antihyperalgesic and antinociceptive effects of peat formulations

PLAMEN IVANOV ZAGORCHEV, ELISAVETA GEORGIEVA APOSTOLOVA, BISSERA ASENOVA PILICHEVA, MILENA NENKOVA DRAGANOVA-FILIPOVA, VESELA YULIEVA KOKOVA and JULIAN BORISOV LUKANOV

apostolova1212@gmail.com

1 Department of Biophysics, Faculty of Pharmacy, Medical University-Plovdiv, 4000 Plovdiv, Bulgaria

2 Department of Pharmacology and Drug Toxicology, Faculty of Pharmacy, Medical University-Plovdiv, 4000 Plovdiv, Bulgaria

3 Department of Pharmaceutical Sciences, Faculty of Pharmacy, Medical University-Plovdiv, 4000 Plovdiv, Bulgaria

4 Department of Medical Biology, Medical Faculty, Medical University-Plovdiv, 4000 Plovdiv, Bulgaria

5 Technological center of emergency medicine, 4000 Plovdiv, Bulgaria

Accepted March 22, 2018

Published online April 27, 2018

 

The aim of this study is to evaluate the antihyperalgesic and antinociceptive effects of two formulations containing peat water extracts using a model of carrageenan-induced hyperalgesia, combined with a test with a mechanical stimulus, and a hot plate test. Rats were divided into seven groups (n = 6) and received local treatment with two peat formulations and two diclofenac formulations dissolved in carbopol gel and Wolff basis creme, respectively. Carbopol gel, Wolff basis creme and 0.9 % NaCl without tested substances were used as controls. Both peat formulations exerted an unambiguous antihyperalgesic effect 60 minutes after the treatment. In the hot plate test, the rats treated with the Wolff basis creme peat formulation showed a tendency to prolonged latency on the first hour. The results could be explained by partial activation of peripheral α2-adrenoceptors and the possible COX-2 suppressive activity.

 

Keywords: peat, hyperalgesia, Wistar rats