Acta Pharm. 67 (2017) 385-395

 

full paper

Short communication

 

Curcumin: Synthesis optimization and in silico interaction with cyclin dependent kinase

MAHMOOD AHMED, MUHAMMAD ABDUL QADIR, MUHAMMAD IMTIAZ SHAFIQ, MUHAMMAD MUDDASSAR, ABDUL HAMEED, MUHAMMAD NADEEM ARSHAD and ABDULLAH M. ASIRI

imtiazshafiqm@yahoo.com

1 Institute of Chemistry, University of the Punjab, Lahore, Pakistan 54590

2 Institute of Biochemistry and Biotechnology, University of the Punjab, Lahore, Pakistan 54590

3 Department of Biosciences, COMSATS Institute of Information Technology, Islamabad- Pakistan

4 H. E. J. Research Institute of Chemistry, International Center for Chemical and Biological Sciences, University of Karachi, Karachi-75270, Pakistan

5 Chemistry Department, Faculty of Science, King Abdulaziz University, Jeddah 21589, Saudi Arabia

6 Center of Excellence for Advanced Materials Research (CEAMR), Faculty of Science, King Abdulaziz University, Jeddah 21589, Saudi Arabia

Accepted April 4, 2017

Published online May 9, 2017

 

Curcumin is a natural product with enormous biological potential. In this study, curcumin synthesis was revisited using different reaction solvents, a catalyst (n-butylamine) and a water scavenger [(n-BuO)3B], to develop the optimal procedure for its rapid acquisition. During synthesis, solvent choice was found to be an important parameter for better curcumin yield and high purity. In a typical reaction, acetyl acetone was treated with boron trioxide, followed by condensation with vanillin in the presence of tri-n-butyl borate as water scavenger and n-butylamine as catalyst at 80 C in ethyl acetate to afford curcumin. Moreover, curcumin was also extracted from turmeric powder and spectroscopic properties such as IR, MS, 1H NMR and 13C NMR with synthetic curcumin were established to identify any impurity. The purity of synthetic and extracted curcumin was also checked by TLC and HPLC-DAD. To computationally assess its therapeutic potential against cyclin dependent kinases (CDKs), curcumin was docked in different isoforms of CDKs. It was observed that it did not dock at the active sites of CDK2 and CDK6. However, it could enter into weak interactions with CDK4 protein.

 

Keywords: curcumin synthesis, CDKs, molecular docking