Acta Pharm. 70 (2020) 89-99


full paper

Short communication


Qualitative and quantitative HPLC-ELSD-ESI-MS analysis of steroidal saponins in fenugreek seed


Department of Pharmacognosy with Medicinal Plants Garden, Medical University of Gdańsk, 80-416 Gdańsk, Poland

Accepted March 19, 2019

Published online April 24, 2019


Fenugreek seeds are known as a source of various compounds, the most common of which are steroidal saponins. However, despite the growing interest in this plant material as a healing agent, spice and dietary supplements ingredient, the composition of Polish fenugreek seeds remains unknown. Therefore, the steroidal saponin complex in the seeds of T. foenum-graecum cultivated in Poland was qualitatively and quantitatively analyzed by the HPLC-ELSD-ESI-MS method. Two C-18 columns connected in a series were used for the first time in analysis of fenugreek saponins and ELS detector parameters were optimized. A total of 26 furostanol saponins were revealed, of which 24 were tentatively identified. The HPLC-ELSD method developed for quantitative analysis was preliminary validated and the determined amount of steroidal saponins in Polish fenugreek seeds was 0.14 %.


Keywords: fenugreek seeds, saponins, HPLC-ELSD-ESI-MS, qualitative and quantitative analysis