Acta Pharm. 71 (2021) 153-162

 

full paper

Short communication

 

In vitro antioxidant and antityrosinase activities of Manilkara kauki

SIRINADA SRISUPAP and CHANYA CHAICHAROENPONG

chanya.c@chula.ac.th

1 Program in Biotechnology, Faculty of Science, Chulalongkorn University, Bangkok 10330, Thailand

2 Institute of Biotechnology and Genetic Engineering, Chulalongkorn University, Bangkok 10330, Thailand

3 Molecular Crop Research Unit, Faculty of Science, Chulalongkorn University, Bangkok 10330, Thailand

Accepted April 3, 2020

Published online April 21, 2020

 

Manilkara kauki L. Dubard is a tropical plant in the genus Manilkara of family Sapotaceae. This study investigated the total phenolic and flavonoid contents, and antioxidant and antityrosinase activities in different parts of M. kauki (fruits, leaves, seeds, stem barks and woods) and in fractions of stem barks. The total phenolic and flavonoid contents of the methanol and aqueous crude extracts of different parts of M. kauki ranged from 10.87 to 176.56 mg GAE (gallic acid equivalents) per gram of crude extract and 14.33 to 821.67 mg QE (quercetin equivalents) per gram of crude extract, resp. Leaves and stem barks exhibited higher total phenolic and flavonoid contents and antioxidant and antityrosinase activities than fruits, seeds and woods. Stem barks were sequentially extracted with n-hexane, ethyl acetate, methanol and water and then the fractionated extracts were subjected to antioxidant and antityrosinase activities testing. The ethyl acetate and methanol extracts of stem barks exhibited higher total phenolic and flavonoid contents and antioxidant and antityrosinase activities than the n-hexane and aqueous extracts. Moreover, ethyl acetate extract of M. kauki stem exhibited the highest antityrosinase activity. It may be a potential source of tyrosinase inhibitors for pharmaceutical and cosmetic applications.

 

Keywords: Manilkara kauki, total phenolic content, total flavonoid content, antioxidant, tyrosinase inhibitor