Acta Pharm. 56 (2006) 1-17

[ Full paper in PDF ]
Review article  
 

Oxidative stress assays for disease risk stratification

DARKO CERNE1* and JANA LUKAC-BAJALO 2

cerne@mf.uni-lj.si

1Medical Centre Ljubljana, Institute for Clinical Chemistry and Biochemistry, 1525 Ljubljana, Slovenia
2 University of Ljubljana, Faculty of Pharmacy, 1000 Ljubljana, Slovenia
Accepted October 11, 2005

Despite the fact that oxidative stress is a significant aetiological factor in several degenerative diseases, its measurement is rarely a part of "routine analyses" performed in hospital clinical chemistry laboratories. This situation is likely to change, as interest in this topic is increasing rapidly. Here we review the pertinent literature, with an assessment to assays for oxidative stress, and categorize them under: (i) assays for monitoring lipid peroxidation, (ii) assays for measuring oxidized amino acids, (iii) assays for measuring oxidized nucleic acids, (iv) assays based on physicochemical and immunological properties of oxidized low-density lipoprotein, and (v) assays for measuring the antioxidant capacity of body fluids and tissues. Our overview should be of help when choosing appropriate laboratory assays for oxidative stress and for routine disease risk stratification.


Keywords: oxidative stress, assay, disease risk stratification