Acta Pharm. 66 (2016) 109-118

 

full paper

Original research paper

 

High performance liquid chromatography for simultaneous determination of xipamide, triamterene and hydrochlorothiazide in bulk drug samples and dosage forms

SOAD S. ABD EL-HAY, HISHAM HASHEM and AYMAN A. GOUDA

sabdulhai@kau.edu.sa

1 Faculty of Pharmacy, Pharmaceutical Chemistry Department, King Abdulaziz University, Saudi Arabia

2 Faculty of Pharmacy, Department of Analytical Chemistry, Zagazig University, Egypt

3 Faculty of Pharmacy, Pharmaceutical Chemistry Department, Jazan University, Saudi Arabia

4 Faculty of Science, Department of Analytical Chemistry, Zagazig University, Egypt

Accepted December 18, 2015

 

A novel, simple and robust high-performance liquid chromatography (HPLC) method was developed and validated for simultaneous determination of xipamide (XIP), triamterene (TRI) and hydrochlorothiazide (HCT) in their bulk powders and dosage forms. Chromatographic separation was carried out in less than two minutes. The separation was performed on a RP C-18 stationary phase with an isocratic elution system consisting of 0.03 mol L1orthophosphoric acid (pH 2.3) and acetonitrile (ACN) as the mobile phase in the ratio of 50:50 at 2.0 mL min1 flow rate at room temperature. Detection was performed at 220 nm. Validation was performed concerning system suitability, limits of detection and quantitation, accuracy, precision, linearity and robustness. Calibration curves were rectilinear over the range of 0.195100 g mL1 for all the drugs studied. Recovery values were 99.9, 99.6 and 99.0 % for XIP, TRI and HCT, respectively. The method was applied to simultaneous determination of the studied analytes in their pharmaceutical dosage forms.

 

Keywords: xipamide, triamterene, hydrochlorothiazide, HPLC, simultaneous analysis