Acta Pharm. 66 (2016) 109-118
Original research paper
High performance liquid chromatography for
simultaneous determination of xipamide, triamterene and hydrochlorothiazide in bulk drug samples
and dosage forms
SOAD S.
ABD EL-HAY, HISHAM HASHEM and AYMAN A. GOUDA
sabdulhai@kau.edu.sa
1 Faculty of Pharmacy, Pharmaceutical Chemistry
Department, King Abdulaziz University, Saudi Arabia
2 Faculty of Pharmacy, Department of Analytical
Chemistry, Zagazig University, Egypt
3 Faculty
of Pharmacy, Pharmaceutical Chemistry Department, Jazan
University, Saudi Arabia
4 Faculty
of Science, Department of Analytical Chemistry, Zagazig
University, Egypt
Accepted December 18, 2015
A novel, simple and robust high-performance liquid chromatography (HPLC) method was developed and validated for simultaneous determination of xipamide (XIP), triamterene (TRI) and hydrochlorothiazide (HCT) in their bulk powders and dosage forms. Chromatographic separation was carried out in less than two minutes. The separation was performed on a RP C-18 stationary phase with an isocratic elution system consisting of 0.03 mol L–1orthophosphoric acid (pH 2.3) and acetonitrile (ACN) as the mobile phase in the ratio of 50:50 at 2.0 mL min–1 flow rate at room temperature. Detection was performed at 220 nm. Validation was performed concerning system suitability, limits of detection and quantitation, accuracy, precision, linearity and robustness. Calibration curves were rectilinear over the range of 0.195–100 µg mL–1 for all the drugs studied. Recovery values were 99.9, 99.6 and 99.0 % for XIP, TRI and HCT, respectively. The method was applied to simultaneous determination of the studied analytes in their pharmaceutical dosage forms.
Keywords: xipamide, triamterene,
hydrochlorothiazide, HPLC, simultaneous analysis