Acta Pharm. 68 (2018) 109-115

 

full paper

Short communication

 

Chronic fluoxetine treatment induces lipid accumulation but does not alter the expression of Pref-1 in adipose tissue of rats

LÁSZLÓ-ISTVÁN BÁBA, ZSOLT GÁLL, ISTVÁN LÓRÁNT BÍRÓ, TIBOR MEZEI, IMRE ZOLTÁN KUN and MELINDA KOLCSÁR

gall.zsolt@umftgm.ro

1 County Hospital of Mures, Tîrgu Mures, Romania

2 Department of Pharmacology and Clinical Pharmacy, University of Medicine and Pharmacy of Tîrgu Mureș, Tîrgu Mureș, Romania

3 Department of Pathology, University of Medicine and Pharmacy of Tîrgu Mureș, Tîrgu Mureș, Romania

4 Doctoral School, University of Medicine and Pharmacy of Tîrgu Mureș, Tîrgu Mureș, Romania

Accepted November 27, 2017

Published online January 25, 2018

 

This study aims to investigate the effects of chronic fluoxetine (FLX) treatment on preadipocyte factor-1 (Pref-1) expression in subcutaneous, visceral and brown adipose tissues, and on the size of vacuoles in adipocytes obtained from the perirenal regions in rats. Twenty-eight Wistar rats were treated with FLX at two different doses and fourteen animals received vehicle. After 40 days of treatment, the subcutaneous, perirenal and interscapular adipose tissues were collected. Pref-1 expression was examined using an immunohistochemical method and the vacuolar area was measured in stained sections. In the low dose FLX group, the size of vacuoles increased both in male and female animals. The high dose of FLX also induced a significant increase of vacuole size, but only in male animals. Neither of the two doses of FLX has significantly affected the Pref-1 expression in any type of adipose tissue.

 

Keywords: fluoxetine, adipose tissue, preadipocyte factor-1, vacuole size