Acta Pharm. 56 (2006) 115-142

Sample preparation and RPHPLC determination of diuretics in human body fluids

DRAGICA ZENDELOVSKA^1* and TRAJCE STAFILOV ²

trajcest@iunona.pmf.ukim.edu.mk

¹Institute of Preclinical and Clinical Pharmacology and Toxicology, St. Cyril and Methodius University, Medical Faculty, 1000 Skopje, Republic of Macedonia
²Institute of Chemistry, Faculty of Science, Sts. Cyril and Methodius University, P.O. Box 162, 1001 Skopje, Republic of Macedonia

This article describes reverse phase high-performance liquid chromatography (RPHPLC) methods for determination of diuretics in different human body fluids (whole blood, plasma, serum or urine). Sample preparation procedures, including solid-phase extraction, liquid-liquid extraction, dilution, precipitation as well as automated RPHPLC procedures, are discussed in order to present the advantages and disadvantages of each type of sample preparation. Also, values of analytical recovery of each procedure used for sample preparation are summarized. The most important RPHPLC parameters (detection mode, stationary phase, mobile phase, sensitivity, etc.) are also summarized and discussed.

Keywords:, diuretics, RPHPLC, determination, human body fluids