Acta Pharm. 64 (2014) 117-129

 

full paper

Original research paper

 

GSTP1, GSTM1 and GSTT1 genetic polymorphisms and total serum GST concentration in stable male COPD

IRENA ŽUNTAR, ROBERTA PETLEVSKI, SLAVICA DODIG and SANJA POPOVIĆ-GRLE

izuntar@pharma.hr

1 University of Zagreb, Faculty of Pharmacy Zagreb, Croatia

2 Children's Hospital Srebrnjak, Referral Centre for Clinical Paediatric Allergology of the Ministry of Health and Social Welfare, Zagreb, Croatia
3 University Hospital for Pulmonary Diseases Jordanovac, Zagreb, Croatia

Accepted October 18, 2013

 

The aim of this study was to test the hypothesis that glutathione-S-transferase (GST) genotypes were associated with COPD. GSTP1, GSTM1 and GSTT1 genotypes were determined by DNA methods and GST activity spectrophotometrically in older male Caucasian Croats (non-smokers, ex-smokers, and smokers) with stable COPD (n = 30) and sex/age matched controls (n = 60). The distribution of GSTP1 genotypes and alleles in controls vs. COPD showed a statistical difference (p < 0.05). The odds ratio of CC/CT+TT (wild type GSTP1 exon 6 vs. joint heterozygous and mutant homozygous GSTP1 exon 6) was 10.000 and statistically different (p = 0.002). In this study, the GSTP1 mutant genotype of exon 5 (GG), as well as GSTP1 mutant and heterozygous genotypes of exon 6 (TT and CT), were suggested to be genetic contributors to COPD susceptibility. Null GSTM1, null GSTT1 and joint GSTM1/GSTT1 null genotypes were not disease associated. Serum GST was not associated with GST genotypes and COPD or smoking history in our study subjects. Conclusions drawn from the study should be further supported and clarified by studies with larger sample sizes.

 

Keywords: glutathione S-transferases (GST), polymorphism, COPD, alpha-1- antitrypsin, lactate dehydrogenase