Acta Pharm. 51 (2001) 159-162

Short communication  

CoCl2 and NiCl2 elicitation and flavonoid production in Ononis arvensis L. culture in vitro


1Department of Pharmacognosy, Faculty of Pharmacy in Hradec Králové, Charles University in Prague,
Hradec Králové, Czech Republic
2Department of Biology, Faculty of Education, University of Hradec Králové, Hradec Králové, Czech Republic
Received January 17, 2000      Accepted March 13, 2001

The abiotic stress can increase the content of secondary metabolites in plants or in plant tissue cultures. The effect of the abiotic elicitors - NiCl2 and CoCl2 on the production of flavonoids in callus culture of Ononis arvensis L. after 24, 48 and 168-hours administration was examined. The tested elicitors markedly influenced the production of flavonoids in this culture. On the basis of our experiments can be concluded that Ni2+ and Co2+ mainly increased flavonoid production in Ononis arvensis L. callus culture mainly after 24 hours. The highest production of flavonoids was observed in the presence of Ni2+(4.2 x 10-7 mol L-1).

Keywords: Ononis arvensis L., flavonoids, elicitation, callus culture