Acta Pharm. 63 (2013) 193-207
Original research paper
Optimization of process variables for
phyllanthin extraction from Phyllanthus
amarus leaves by supercritical
fluid using a Box-Behnken experimental design followed by HPLC identification
AJIT A.
PATIL, SACHIN S. BHUSARI, DEVANAND B. SHINDE and PRAVIN S. WAKTE
pharmtech.udct@gmail.com
Pharmaceutical Technology Division, Department of
Chemical Technology, Dr. Babasaheb Ambedkar Marathwada University, Aurangabad-431004,
India
Accepted January 28, 2013
The response surface methodology using the Box-Behnken design was established to describe supercritical carbon dioxide assisted extraction of phyllanthin from Phyllanthus amarus Schum and Thonn leaves prior to HPLC analysis. The effects of extraction pressure, temperature, modifier concentration and extraction time on the yield of phyllanthin were investigated. By solving the regression equation, the optimum conditions were as follows: extraction pressure 23.2 MPa, temperature 40 °C, methanol as modifier at a concentration 10 % and time 90 min. Under these conditions, the phyllanthin yield was 12.83 ± 0.28 mg g-1, which was in good agreement with the predicted values. Modifier concentration and extraction time showed a significant effect on the phyllanthin yield.
Keywords: phyllanthin, Phyllanthus
amarus (Euphorbiaceae),
supercritical carbon dioxide assisted extraction, Box-Behnken design, HPLC