Acta Pharm. 60 (2060) 197-205

full paper

Short communication

Validated RP-HPLC and TLC methods for simultaneous estimation of tamsulosin hydrochloride and finasteride in combined dosage forms

DIPTI B. PATEL and NATUBHAI J. PATEL

diptibpatel_24980@yahoo.co.in

S. K. Patel College of Pharmaceutical Education and Research, Department of Pharmaceutical Chemistry, Ganpat University, Kherva, Mehsana, Gujarat, India-382711

Accepted February 2, 2010

Reversed phase high-performance liquid chromatography (RP-HPLC) and thin-layer chromatography (TLC) methods have been developed and validated for simultaneous estimation of tamsulosin hydrochloride and finasteride in bulk drug and in combined dosage forms. RP-HPLC separation was achieved on a Phenomenex C₁₈ column using methanol/0.02 mol L^-1 ammonium acetate buffer/triethylamine (79.9 + 20 + 0.1, V/V/V) (pH 9.2) as mobile phase. The TLC separation was achieved on an aluminium-backed layer of silica gel 60F₂₅₄ using toluene/methanol/triethylamine (9 + 1.5 + 1, V/V/V) as eluent. Quantification was achieved with photodiode array (PDA) detection at 235 nm over the concentration range 0.5 16 and 1-50 g mL^-1 with mean recovery of 99.8 0.9 and 100.0 0.8 % for tamsulosin hydrochloride and finasteride, respectively, by the RP-HPLC method. Quantification was achieved with UV detection at 270 nm over the concentration range 100 2000 ng per spot and 250 5000 ng per spot with mean recovery of 98.9 0.9 and 99.6 0.7 % for tamsulosin hydrochloride and finasteride, respectively, by the TLC method. Both methods are simple, precise, accurate and sensitive and are applicable to the simultaneous determination of tamsulosin hydrochloride and finasteride in bulk drug and in combined dosage forms.

Keywords: tamsulosin hydrochloride, finasteride, RP-HPLC, TLC