Acta Pharm. 57 (2007) 231-239
Micellar liquid chromatographic analysis of benzyl alcohol and benzaldehyde in injectable formulations
MOHAMED RIZK, FAWZIA IBRAHIM, MOHAMED HEFNAWY and JENNY JEEHAN NASR*
Faculty of Pharmacy, Department of Analytical Chemistry, University of Mansoura, Mansoura-35510, Egypt
Accepted February 5, 2007
An accurate, sensitive and selective reversed-phase micellar liquid chromatographic method was developed for simultaneous determination of benzyl alcohol and benzaldehyde. This method was applied in different injectable formulations containing diclofenac, piroxicam. lincomycin and clindamycin. The method showed excellent linearity in the range of 10-100 and 1-20 μg mL-1 with the limit of detection (S/N = 3) 0.25 μg mL (2.3 × 10-6 mol L-1) and 0.12 µg mL-1 (1.13 × 10-6 mol L-1) for benzyl alcohol and benzaldehyde, respectively. The suggested method was successfully applied for the analysis of the studied drugs in bulk with average recoveries of 100.1 ± 1.0% for benzyl alcohol and 100.4 ± 1.6% for benzaldehyde and to the determination of benzyl alcohol and benzaldehyde in injectable formulations with the respective average recoveries of 99.8 ± 0.3 and 100.0 ± 0.4%.
Keywords: micellar liquid chromatography, benzyl alcohol, benzaldehyde, injectable formulations