Acta Pharm. 65 (2015) 235-252
Original research paper
Dissolution
profiles of perindopril and indapamide
in their fixed-dose formulations by a new HPLC method and different
mathematical approaches
ANNA
GUMIENICZEK, PAULINA MĄCZKA, ŁUKASZ KOMSTA and RAFAŁ PIETRAŚ
anna.gumieniczek@umlub.pl
Department
of Medicinal Chemistry, Faculty of Pharmacy, Medical University in Lublin,
20-090 Lublin, Poland
Accepted May 14, 2015
A new HPLC method was introduced and validated for simultaneous determination of perindopril and indapamide. Validation procedure included specificity, sensitivity, robustness, stability, linearity, precision and accuracy. The method was used for the dissolution test of perindopril and indapamide in three fixed-dose formulations. The dissolution procedure was optimized using different media, different pH of the buffer, surfactants, paddle speed and temperature. Similarity of dissolution profiles was estimated using different model-independent and model-dependent methods and, additionally, by principal component analysis (PCA). Also, some kinetic models were checkedwith dissolved amounts of drugs as a function of time.
Keywords: perindopril, indapamide, dissolution
profiles, model-independent methods, model-dependent methods, PCA