Acta Pharm. 52 (2002) 243-250

Original research paper  
 

Simultaneous quantification of cefaclor and cephalexine in blood plasma using high-performance liquid chromatography with UV detection

DRAGICA ZENDELOVSKA,1 TRAJCE STAFILOV2*  and   STOJMIR PETROV1

trajcest@iunona.pmf.ukim.edu.mk

1Institute of Preclinical and Clinical Pharmacology and Toxicology, Sts. Cyril and Methodius University,
Medical Faculty, Skopje, Republic of Macedonia
2Institute of Chemistry, Faculty of Science, Sts. Cyril and Methodius University, Skopje, Republic of Macedonia
Received September 27, 2002      Accepted November 11, 2002

Concentration of cefaclor and cephalexine were measured simultaneously in blood plasma after protein precipitation, using a newly developed RP high-performance liquid chromatographic method on C8 colum. The method was found to be reproducible with a relative standard deviation less than 6.0% over the concentration range 0.2-30.0 mg mL-1 for cefaclor and 0.5-50.0 mg mL-1 for cephalexine in blood plasma samples. The limits of quantification were 0.1 and 0.25 mg mL-1, respectively.


Keywords: cefaclor, cephalexine, high-performance liquid chromatography