Acta Pharm. 67 (2017) 247-256
Short communication
Testing of resveratrol
microemulsion photostability
and protective effect against UV induced oxidative stress
VAIDA
JUŠKAITĖ, KRISTINA RAMANAUSKIENĖ and VITALIS BRIEDIS
Lithuanian University of Health
Sciences, Department of Clinical Pharmacy, Kaunas, Lithuania
Accepted February 26, 2017
Published online May 4,
2017
Resveratrol is
well known for its antioxidant activity and susceptibility to ultraviolet
radiation. Development of formulations providing improved stability and
relevant drug delivery of resveratrol is still a
challenging task. The aim of this study was to determine protective
characteristics of formulated microemulsions by
evaluating photoisomerization of resveratrol
and to investigate the effects of resveratrol on
human keratinocyte cells under oxidative stress
caused by ultraviolet radiation. Incorporation of resveratrol
into microemulsions resulted in increased photostability of active compounds and the results
demonstrated that photodegradation
of resveratrol was significantly delayed. Results of
biopharmaceutical evaluation in vitro
demonstrated that up to 60 % of resveratrol was
released from microemulsions within 6 hours under a constant
release rate profile. In vivo biological
testing confirmed the ability of resveratrol to
protect cells from oxidative stress and to increase cell viability. It was
concluded that microemulsions might be considered in the
development of UV light sensitive compounds.
Keywords: microemulsion, resveratrol, photostability, HaCaT cells, UVB,
oxidative stress