Acta Pharm. 58 (2008) 275-285
Original research paper
Development and validation of
spectrophotometric methods for determination of ceftazidime in pharmaceutical
dosage forms
BASAVARAJ HIREMATH and BENNIKALLU
HIRE MATHADA MRUTHYUNJAYASWAMY
Department of Chemistry,
Gulbarga University, Gulbarga-585 106, India
Accepted June 30, 2008
Two spectrophotometric methods for the determination of ceftazidime
(CFZM) in either pure form or in its pharmaceutical formulations are described.
The first method is based on the reaction of 3-methylbenzothiazolin-2-one
hydrazone (MBTH) with ceftazidime in the presence of ferric chloride in acidic
medium. The resulting blue complex absorbs at λmax 628 nm. The second method describes the
reaction between the diazotized drug and N-(1-naphthyl)ethylenediamine
dihydrochloride (NEDA) to yield a purple colored product with λmax at 567 nm. The
reaction conditions were optimized to obtain maximum color intensity. The
absorbance was found to increase linearly with increasing the concentration of
CFZM; the systems obeyed the Beer’s law in the range 2-10 and 10-50 µg mL-1 for MBTH and NEDA methods, respectively. LOD, LOQ and correlation
coefficient values were 0.15, 0.79 and 0.50, 2.61. No interference was observed
from common excipients present in pharmaceutical formulations. The proposed
methods are simple, sensitive, accurate and suitable for quality control
applications.
Keywords: ceftazidime, diazotization,
spectrophotometry, pharmaceutical formulation