Acta Pharm. 49 (1999) 29–34

Original scientific paper  

Influence of cAMP on differentiation of erythroid cells


Division of Molecular Medicine,  Rudjer Boškovic Institute,  Bijenicka 54,  10000 Zagreb,  Croatia
Received  October 28, 1998      Accepted  January 25, 1999

Adenosine 3',5'-cyclic monophosphate (cAMP), a mediator of intracellular signal, plays an important role in controlling cell proliferation and differentiation. In some animal cells an increase in cAMP activates the transcription of specific genes.
The aim of this study has been to examine the influence of cAMP on the events during erythroid differentiation. The chicken erythroblast cell line (HD3), transformed with temperature sensitive erythroleukemia virus (ts34), was used as a model. The cells were induced to differentiate and the markers of the erythroid differentiation were measured. The specific inhibitors, which modulate the intracellular level of cAMP, were also used.
Differentiation of the HD3 cells is followed by increasing the level of intracellular cAMP. This elevation is an important event involved in the terminal differentiation of the HD3 cells, but probably not compatible with differentiation itself. In comparison with hemin and butyric acid, 3-i-butyl-1-methyl-xanthyne, a cAMP elevating agent decreased the synthesis of haemoglobin. This suggests that a high level of intracellular cAMP could have a negative influence on differentiation of the HD3 cells.

Keywords: HD3 cells, differentiation, cAMP level