Acta Pharm. 59 (2009) 325-334
Sterodin®, a novel immunostimulating drug: Some toxicological and pharmacological evaluations in vivo, and drug-lipid interaction studies in vitro
SARBANI DEY RAY, PARTHA PRATIM ROY and SUPRATIM RAY
1 Dr. B C Roy College of Pharmacy & Allied Health Sciences, Bidhannagar, Durgapur-713206, India
2 Division of Pharmaceutical Chemistry, Department of Pharmaceutical Technology, Jadavpur University, Kolkata-700032, India
Accepted July 20, 2009
SterodinÒ is a novel non-specific immunostimulating drug produced by combination of bile lipids and bacterial metabolites. In the present study, we investigated some of its (i) toxicological and (ii) pharmacological properties in vivo, and (iii) drug-lipid interaction (lipid peroxidation) in vitro. We also evaluated the possible (iv) SterodinÒ-induced lipid peroxidation as well as the effect of ascorbic acid on this peroxidation. We found LD50 of SterodinÒ to be 31.50 mL kg-1 body mass. In male albino mice, Sterodin increased the total white blood cells and neutrophils count by 59 and 26 %, respectively, on the 6th day compared to day 0 after injection and stimulated phagocytic activity in vivo. We used goat liver as lipid source in drug-lipid interaction studies in vitro. Our experiments show that SterodinÒ induces lipid peroxidation, which was prevented by ascorbic acid.
Keywords: SterodinÒ, immunostimulating drug, ascorbic acid, lipid peroxidation, malondialdehyde, reduced glutathione