Acta Pharm. 64 (2014) 325-334
Original research paper
Analysis of active components in Salvia miltiorrhiza injection based on vascular
endothelial cell protection
JIE SHEN,
KAI YANG, CAIHUA SUN and MINXIA ZHENG
slightsusu@163.com
1 The
First Affiliated Hospital of Zhejiang Chinese Medical University, Hangzhou,
Zhejiang Province – 310 006, China
2 The
First Affiliated Hospital of Medical School of Zhejiang University, Hangzhou,
Zhejiang Province – 310 003, China
Accepted May 14, 2014
Correlation analysis based on chromatograms and pharmacological activities is essential for understanding the effective components in complex herbal medicines. In this report, HPLC and measurement of antioxidant properties were used to describe the active ingredients of Salvia miltiorrhiza injection (SMI). HPLC results showed that tanshinol, protocatechuic aldehyde, rosmarinic acid, salvianolic acid B, protocatechuic acid and their metabolites in rat serum may contribute to the efficacy of SMI. Assessment of antioxidant properties indicated that differences in the composition of serum powder of SMI caused differences in vascular endothelial cell protection. When bivariate correlation was carried out, it was found that salvianolic acid B, tanshinol and protocatechuic aldehyde were the active components of SMI because they were correlated to antioxidant properties.
Keywords: Salvia miltiorrhiza
injection, HPLC, antioxidant activity, correlation analysis, salvianolic acid