Acta Pharm. 54 (2004) 331-338

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Original research paper  
 

Healing potential of Anogeissus latifolia for dermal wounds in rats

RAGHAVAN GOVINDARAJAN,1  MADHAVAN VIJAYAKUMAR,1  CHANDANA VENKATESHWARA RAO,1 ANNIE SHIRWAIKAR,2  SHANTA MEHROTRA1* and PALPU PUSHPANGADAN1

govind108@yahoo.com

1Pharmacognosy and Ethnopharmacology Division, National Botanical Research Institute, Lucknow - 226 001, India
2Pharmacognosy Department, College of Pharmaceutical Sciences, Manipal - 576 119, Karnataka, India
Received June 22, 2003      Accepted November 24, 2004

Wound healing potential of etnanolic extract of Anogeissus latifolia bark (ALE) for treatment of dermal wounds in rats was studied on excision and incision wound models. HPTLC of the total extract was recorded for the purpose of standardization. Various parameters of incision wound, viz. epithelization period, scar area, tensile strength and hydroxyproline measurements along with wound contraction, were used to evaluate the effect of A. latifolia on wound healing. The results obtained indicate that A. latifolia accelerates the wound healing process by decreasing the surface area of the wound and increasing the tensile strength. Nitrofurazone ointment was used as a positive control. Complete epithelization was observed within 15 days with ALE. Measurements of the healed area and the hydroxyproline level were in agreement. Antibacterial activity of ALE was studied against Gram-positive (Staphylococcus aureus) and Gram-negative bacteria (Escherichia coli, Pseudomonas aeruginosa and Klebsiella pneumoniae) compared to erythromycin and tetracycline. Moderate activity was observed against all organisms. The present study provides a scientific rationale for the traditional use of Anogeissus latifolia in the management of skin diseases such as sores, boils and itching.


Keywords: Anogeissus latifolia (Combretaceae), wound healing, antibacterial activity, HPTLC