Acta Pharm. 69 (2019) 33-48

 

full paper

Original research paper

 

Therapeutic bullfrog oil-based nanoemulsion for oral application: Development, characterization and stability

RENATA RUTCKEVISKI, FRANCISCO HUMBERTO XAVIER, ANDREZA ROCHELLI DO VALE MORAIS, LUCAS AMARAL-MACHADO, EVERTON DO NASCIMENTO ALENCAR, JULIETA GENRE, ADRIANO ANTUNES DE SOUZA ARAUJO, ERYVALDO SOCRATES TABOSA DO EGITO

socrates@ufrnet.br, socratesegito@gmail.com

1 Universidade Federal do Rio Grande do Norte (UFRN), Programa de Pós-graduação em Ciências Farmacêuticas, Departamento de Farmácia, 59010-180, Natal–RN, Brazil

2 UFRN, Laboratório de Sistemas Dispersos (LaSiD), Departamento de Farmácia, 59010-180, Natal–RN, Brazil

3 UFRN, Programa de Pós-graduação em Nanotecnologia Farmacêutica, LaSiD, Departamento de Farmácia, 59010-180, Natal–RN, Brazil

4 UFRN, Programa de Pós-graduação em Ciências da Saúde, LaSiD, Departamento de Farmácia, 59010-180, Natal–RN, Brazil

5 Universidade Federal de Sergipe, Departamento de Farmácia. 49100-000, São Cristovão–SE, Brazil

Accepted July 9, 2018

Published online August 29, 2018

 

The aim of this study was to develop, optimize, and characterize a stable therapeutic bullfrog oil based nanoemulsion for oral application using a rational experimental design approach. The optimized oral nanoemulsion contained 0.2 % sodium benzoate and 0.02 % propyl-paraben as preservatives; 0.1 % sucralose and 0.4 % acesulfam K as sweeteners and 0.1 % tutti-frutti as flavoring to mask the unpleasant organoleptic characteristics of the bullfrog oil. The oral O/W-nanoemulsion showed droplet size, PDI, zeta potential, and pH of 410 ± 8nm, 0.20 ± 0.02, –38 ± 2.5 mV and 6.43 ± 0.05, respectively. The optimized oral nanoemulsion presented a milky single-phase and an optimal physical stability at 25 °C for 90 days. Indeed, the higher oxidation induction time and the lower formation of peroxides in the oral nanoemulsion were responsible for improving its stability. A therapeutic delivery system containing bullfrog oil for oral application was successfully developed and optimized with ideal thermo-oxidative stability.

 

Keywords: bullfrog oil, nanoemulsion, thermo-oxidative stability, experimental design, oral application, therapeutic nanosystem