Acta Pharm. 57 (2007) 333-342
10.2478/v10007-007-0026-4
Original research paper
A sensitive spectrophotometric
method for the determination of sulfonamides in pharmaceutical preparations
PADMARAJAIAH
NAGARAJA1*, SHAILENDRA D. NAIK2, ASHWINEE
KUMAR SHRESTHA1 and ANANTHARAMAN SHIVAKUMAR1
nagarajap58@yahoo.co.in
1 Department
of Studies in Chemistry, University of Mysore, Manasagangothri, Mysore-570006, India
2 Charak Pharma Pvt. Ltd., Silvassa, U. T.
of Dadra Nagar Haveli, Silvassa-396230, India
Accepted June 26, 2007
A
new, simple and sensitive spectrophotometric method
for the determination of some sulfonamide drugs has been developed. The method
is based on the diazotization of sulfacetamide,
sulfadiazine, sulfaguanidine, sulfamerazine,
sulfamethazine, sulfamethoxazole
and their coupling with 8-hydroxyquinoline in alkaline
media to yield red coloured products, with absorption
maximum at 500 nm. The Beer s law is obeyed from 0.1-7.0 g mL-1. The limits of quantification and limits of detection were 0.11-0.18 and 0.03-0.5 g mL-1, respectively. Intraday
precision (RSD 0.1-0.5%) and accuracy (recovery 97.3-100.8) of
the developed method were evaluated. No interference was observed from common adjuvants. The method has been successfully applied to the
assay of sulpha drug in the pharmaceutical
formulations.
Keywords: sulfonamide drugs, diazotisation, 8-hydroxyquinoline,
spectrophotometry, pharmaceutical formulation