Acta Pharm. 49 (1999) 35–41
Cryoprecipitate with heparin was the starting material in chromatographic isolation of factor VIII (F VIII). Heparin stabilizes F VIII and in that sense enhances the recovery of F VIII during isolation. The heparin concentration during different steps of F VIII isolation procedure was determined by modified azure A method. Azure A method is a rapid and simple spectrometric method for determination of heparin following the formation of soluble complex between heparin and azure A dye. Influence of different parameters (pH, heparin concentration, stability of the dye and heparin-dye complex) on azure A method has been established. Results presented in this report show that determination of heparin is achieved by simultaneous measuring absorbance of the complex at 500 nm and azure A dye absorbance decrease at 630 nm.
Keywords: F VIII, heparin, azure