Acta Pharm. 57 (2007) 371-377

10.2478/v10007-007-0030-8

full paper

Short communication

Assay of flavonoid aglycones from species of the genus Sideritis (Lamiaceae) from Macedonia with HPLC‑UV DAD

BISERA JANESKA, MARINA STEFOVA and KALINA ALIPIEVA

marinaiv@iunona.pmf.ukim.edu.mk

¹Institute of Chemistry, Faculty of Science, Sts. Cyril and Methodius University, Skopje, Republic of Macedonia

²Institute of Organic Chemistry with Centre of Phytochemistry, Bulgarian Academy ofSciences, Sofia, Bulgaria

Accepted March 9, 2007

Flavonoids obtained from Sideritis species (Lamiaceae), S. raeseri and S. scardica, grown in Macedonia were studied. Qualitative and quantitative analyses of the flavonoid aglycones were performed using high-performance liquid chromatography (HPLC) with a UV diode array detector. Extracts were prepared by acid hydrolysis in acetone, reextraction in ethyl acetate and evaporation to dryness; the residue dissolved in methanol was subjected to HPLC analysis.

Isoscutellarein, chryseriol and apigenin were identified in the extracts. Also, a 4 -methyl ether derivative of isoscutellarein was found, together with hypolaetin and its methyl ether derivative, which were identified according to previously isolated glycosides and literature data. Quantitation was performed using calibration with apigenin.

According to this screening analysis, the samples of the genus Sideritis from Macedonia are rich in polyhydroxy flavones and analogous with the previously studied Mediterranean Sideritis species from the Ibero-North African and Greek Sideritis species with respect to the presence of 8-OH flavones and their derivatives.

Keywords: flavonoids, polyhydroxy flavones, Sideritis, HPLC-UV DAD