Acta Pharm. 62 (2012) 371-382

 

full paper

Original research paper

 

In vitro antioxidant activity of stem bark of Trichilia catigua Adr. Juss

JEAN PAUL KAMDEM, SÍLVIO TERRA STEFANELLO, ALINE AUGUSTI BOLIGON, CAROLINE WAGNER, IGE JOSEPH KADE, ROMAIANA PICADA PEREIRA, ALESSANDRO DE SOUZA PRESTE, DANIEL HENRIQUE ROOS, EMILY PANSERA WACZUK, ANDRE STORTI APPEL, MARGARETH LINDE ATHAYDE, DIOGO ONOFRE SOUZA and JOÃO BATISTA TEIXEIRA ROCHA

jbtrocha@yahoo.com.br; kamdemjeanpaul2005@yahoo.fr

1 Departamento de Química, Programa de Pós-Graduação em Bioquímica Toxicológica, Universidade Federal de Santa Maria, Santa Maria, RS 97105-900, Brazil

2 Departamento de Farmácia Industrial, Programa de Pós-Graduação em Ciências Farmacêuticas, Universidade Federal de Santa Maria, Santa Maria, RS 97105-900, Brazil

3 Universidade Federal do Pampa, Campus Caçapava do Sul, Caçapava do Sul, RS CEP 96570-000, Brazil

4 Department of Biochemistry, Federal University of Technology, PMB 704 Akure, Ondo State, Nigeria

5 Departamento de Bioquímica, Instituto de Ciências Básicas da Saúde, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brazil

Accepted June 11, 2012

 

Antioxidant activity of the ethanolic extract and fractions from the stem bark of T. catigua was investigated. IC50 (for DPPH scavenging) by T. catigua varied from 9.17 ± 0.63 to 76.42 ± 5.87 μg mL-1 and total phenolic content varied from 345.63 ± 41.08 to 601.27 ± 42.59 µg GAE g-1 of dry extract. Fe2+-induced lipid peroxidation was significantly reduced by ethanolic extract and fractions. Mitochondrial Ca2+-induced dichlorofluorescein oxidation was significantly reduced by the ethanolic extract in a concentration-dependent manner. Ethanolic extract reduced mitochondrial Δψm only at high concentrations (40-100 μg mL-1), which indicates that its toxicity does not overlap with its antioxidant effects. Results suggest involvement of antioxidant activities of T. catigua in its pharmacological properties.

 

Keywords: Trichilia catigua, antioxidant, flavonoids, phenolics, reactive oxygen species, oxidative stress