Acta Pharm. 69 (2019) 413-422


full paper

Original research paper


Comparison of high-performance thin layer chromatography/UV-densitometry and UV-derivative spectrophotometry for determination of trimetazidine in pharmaceutical formulation


1 Department of Toxicology, Faculty of Pharmacy, Collegium Medicum of Nicolaus Copernicus University, Bydgoszcz, Poland

2 Department of Geriatrics, Collegium Medicum in Bydgoszcz, Nicolaus Copernicus University in Toruń, 85-094 Bydgoszcz, Poland

3 Medical Physics Division, Biophysics Department, Faculty of Pharmacy, Collegium Medicum, Nicolaus Copernicus University, Bydgoszcz, Poland

Accepted January 21, 2019

Published online February 11, 2019


New methods for assaying trimetazidine dihydrochloride on the basis of thin layerNew methods for assaying trimetazidine dihydrochloride on the basis of thin layer chromatography and spectrophotometry are proposed and compared in the paper. In HPTLC/UV-densitometry, separation is achieved by using a mobile phase composed of ammonia-methanol (30:70, V/V) on silica gel HPTLC plates F254. Quantification using a non-linear calibration curve is accomplished by densitometric detection at 230 nm. Derivative spectrophotometric determination of trimetazidine dihydrochloride is carried out from the fourth derivative of the absorbance at 233 nm in peak-zero mode. Statistical comparison led to the conclusion that there is no significant difference between the two studied methods and, moreover, that they demonstrate satisfactory accuracy and precision for routine applications.


Keywords: trimetazidine dihydrochloride, high performance thin layer chromatography/UV-densitometry, derivative spectrophotometry, pharmaceutical formulations