Acta Pharm. 59 (2009) 421-429

 

full paper

Original research paper

 

Development of a radiolabeled β-human chorionic gonadotropin

AMIR R. JALILIAN, MOHAMMAD REZA KHOSHDEL, JAVAD GAROUSI, HASSAN YOUSEFNIA, MOHAMMAD A. HOSSEINI, SAEED RAJABIFAR and DARYOUSH SARDARI

ajalilian@nrcam.org

1Nuclear Medicine Research Group, Agricultural, Medical and Industrial Research School (AMIRS-NSTRI), Karaj, Iran

2Islamic Azad University, Science and Research Branch, P. O. Box 14155-775, Tehran, Iran

3Islamic Azad University, Young Researcher Club, Takestan, Branch, Iran

Accepted September 8, 2009

 

b-human chorionic gonadotropin (b-hCG) was successively labeled with [67Ga] gallium chloride after conjugation with freshly prepared diethylenetriaminepentaacetic acid dianhydride (ccDTPA). After solid phase purification of the radiolabeled hormone, high performance liquid chromatography showed radiochemical purity higher than 95 % under optimized conditions (specific activity = 22-23 TBq mM-1, labeling efficiency 80 %). Preliminary in vivo studies (ID g-1, %) in male wild-type rats showed marked gonadal uptake of the tracer after 240 minutes in agreement with the biodistribution studies and reported b-hCG receptors. Target to blood ratios were 5.1 and 15.2 after 3 and 24 hours, respectively, while target to muscle ratios were 35 and 40 after 3 and 24 hours, respectively.

 

Keywords: b-hCG, radiolabeling, biodistribution, radiopharmaceutical