Acta Pharm. 66 (2016) 443-448

 

full paper

Short communication

 

Effect of cerebrolysin on dopaminergic neurodegeneration of rat with oxidative stress induced by 3-nitropropionic acid

DAVID CALDERÓN GUZMÁN, NORMA OSNAYA BRIZUELA, MARIBEL ORTÍZ HERRERA, ERNESTINA HERNÁNDEZ GARCÍA, GERARDO BARRAGÁN MEJÍA, HUGO JUÁREZ OLGUÍN, ARMANDO VALENZUELA PERAZA, JONAS ATTILUS and NORMA LABRA RUÍZ

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1 Laboratorio de Neurociencias, Instituto Nacional de Pediatría (INP), México City, México

2 Laboratorio de Bacteriología Experimental, INP, México City, México

3 Laboratorio de Farmacología, INP, México City, México

Accepted March 8, 2016

Published online May 16, 2016

 

The study tested the hypothesis that cerebrolysin protects the brain from free radicals in rats treated with 3-nitropropionic acid (3-NPA). To address this hypothesis, the levels of dopamine (DA) and some oxidative stress biomarkers were measured after administration of 3-NPA. Young male Fischer rats were treated for three days with cerebrolysin, 3-NPA or both substances. Their brains were extracted, and DA, lipid peroxidation (LP), glutathione (GSH), calcium, and H2O2 were measured using validated methods. In the cortex, hemispheres and cerebellum/medulla oblongata of the group treated with cerebrolysin and 3-NPA, the levels of DA and LP decreased. In addition, calcium and H2O2 levels decreased in the hemispheres of the same group, while GSH increased in cortex. The increased dopamine metabolism due to the administration of cerebrolysin led to increased formation of radical species and oxidative stress, especially when free radicals were generated by 3-NPA.

 

Keywords: cerebrolysin, dopamine, 3-nitropropionic acid, oxidative stress