Acta Pharm. 61 (2011) 457-463
Short communication
Assay of lercanidipine hydrochloride in dosage
forms using nucleophilic substitution reaction
THUTTAGUNTA
MANIKYA SASTRY and KARIPEDDI RAMAKRISHNA
1 Department
of Chemistry, G V P College of Engineering, Visakhapatnam-530047, India
2 Department of Chemistry, Institute of Science,
GITAM University, Visakhapatnam-530045, India
Accepted August 3, 2011
A simple and sensitive spectrophotometric method has been developed for the assay of lercanidipine hydrochloride (LER) in bulk and in formulations. The method is based on the formation of coloured species between the drug and 1,2-naphthaquinone-4-sulphonic acid sodium salt (NQS) by means of nucleophilic substitution reaction. The absorbance was measured at lmax = 460. The method was analyzed statistically. The system obeyed the Beer’s law in the range 20-100 mg mL-1. Molar absorptivity value was found to be 4.79 × 103 L mol-1 cm-1. Limits of detection and quantification were found to be as low as 0.04 and 0.13 mg mL-1. Precision (RSD, 0.4 %) and accuracy (recovery 99.2 ± 0.6 to 101.1 ± 0.8 %) of the developed method were evaluated.
Keywords: spectrophotometry, lercanidipine hydrochloride,
1,2-naphthaquinone-4-sulphonic acid sodium salt (NQS)