Acta Pharm. 64 (2014) 475-484

 

full paper

Short communication

 

Evaluation of anti-Bothrops asper venom activity of ethanolic extract of Brownea rosademonte leaves

MARCOS SALAZAR, LILIA CHÉRIGO, HILDAURA ACOSTA, RAFAEL OTERO and SERGIO MARTÍNEZ-LUIS

smartinez@indicasat.org.pa

1 Centro de Investigación e Información de Medicamentos y Tóxicos (CIIMET), Facultad de Medicina, Universidad de Panamá

2 Facultad de Ciencias Naturales, Exactas y Tecnología, Universidad de Panamá

3 Facultad de Medicina, Universidad de Antioquia, Medellín, Colombia

4 Center for Biodiversity and Drug Discovery, INDICASAT-AIP, Panama, Panama

Accepted June 20, 2014

 

Significant inhibition of the coagulant and hemorrhagic effects of Bothrops asper venom was demonstrated by ethanolic extract prepared from the leaves of Brownea rosademonte. In vitro experiments preincubating 5.5 mg extract kg–1 b.m. for 30 min with a minimum hemorrhagic dose of venom (273.8 ± 16.1 µg of venom kg–1 b.m.) lowered the hemorrhagic activity of the venom in CD-1 mice by 51.5 ± 2.6 %. Additionally, 1.7 mg extract L–1 plasma prolonged 5.1 times the plasma coagulation time. Fractionation of the extract led to isolation of two compounds: ononitol (1) and quercetrin (2). The structure of compounds 1 and 2 was established by spectroscopic analyses, including APCI-HR-MS and NMR (1H, 13C, HSQC, HMBC and COSY). Quercetrin prolonged 2.6 times the plasma coagulation time demonstrating that this compound was one of the active constituents of the Brownea rosademonte extract.

 

Keywords: Bothropsasper (Viperidae) venom, Brownea rosademonte (Caesalpinaceae), quercetrin, anticoagulant, antihemorrhagic, antiproteolytic, antihemolytic