Acta Pharm. 58 (2008) 479-487

full paper

Short communication

 

Effect of ampicillin and chloroquine on humoral immune response elicited by bovine albumin encapsulated in liposomes

JITENDER MADAN, DINESH KAUSHIK, SATISH SARDANA, DINA NATH MISHRA, SHALINDER KUMAR SINGH and BINOD KUMAR SINGH

sardanasatish@gmail.com

Department of Pharmaceutics, Hindu College of Pharmacy, Sonepat (Haryana), India

Department of Pharmaceutical Sciences, Guru Jambheshwar University of Science and Technology, Hisar (Haryana) India

Department of Pharmacy, Kumaon University, Nainital, India

Accepted October 24, 2008

 

Immune suppression resulting from chemoprophylaxis and potential drug interaction were investigated in experimental animals pre-medicated with ampicillin and chloroquine followed by immunization with bovine serum albumin bearing liposomes prepared by the reverse phase evaporation method. The prepared liposomes were evaluated for particle size, entrapment efficiency and in vitro release. Humoral immune response was measured in terms of systemic IgG antibody titre by the ELISA method. The present study showed that 7:3 molar ratio of soya phosphatidylcholine and cholesterol produced liposomes of mean diameter of 235.4 10.3 nm and entrapment efficiency of 41.3 3.2%. Ampicillin significantly (p < 0.05) decreased the antibody titre whereas chloroquine did not reduce the antibody titre significantly. The study will help in programming a new drug management and in characterization of vaccine-drug interaction.

 

Keywords: immunomodulation, liposomes, bovine albumin, ampicillin, chloroquine