Acta Pharm. 58 (2008) 479-487
Short communication
Effect of ampicillin
and chloroquine on humoral immune response elicited by bovine albumin
encapsulated in liposomes
JITENDER MADAN, DINESH KAUSHIK, SATISH SARDANA,
DINA NATH MISHRA, SHALINDER KUMAR SINGH and BINOD KUMAR SINGH
sardanasatish@gmail.com
Department of
Pharmaceutics, Hindu
Department of
Pharmaceutical Sciences, Guru Jambheshwar University of Science and Technology,
Hisar (Haryana)
Department of Pharmacy,
Accepted October 24, 2008
Immune suppression resulting from chemoprophylaxis and potential
drug interaction were investigated in experimental animals pre-medicated with
ampicillin and chloroquine followed by immunization with bovine serum albumin
bearing liposomes prepared by the reverse phase evaporation method. The
prepared liposomes were evaluated for particle size, entrapment efficiency and in
vitro release. Humoral immune response was measured in terms of systemic
IgG antibody titre by the ELISA method. The present study showed that 7:3 molar
ratio of soya phosphatidylcholine and cholesterol produced liposomes of mean
diameter of 235.4 ± 10.3 nm and entrapment efficiency of
41.3 ± 3.2%. Ampicillin significantly (p < 0.05) decreased the antibody
titre whereas chloroquine did not reduce the antibody titre significantly. The
study will help in programming a new drug management and in characterization of
vaccine-drug interaction.
Keywords: immunomodulation, liposomes, bovine albumin, ampicillin,
chloroquine