Acta Pharm. 66 (2016) 503-514
Original research paper
Ionophore-based potentiometric
PVC membrane sensors
for determination of phenobarbitone in its
pharmaceutical formulations
HAITHAM ALRABIAH, ABDULRAHMAN AL-MAJED, MOHAMMED
ABOUNASSIF and GAMAL A. E. MOSTAFA
1 Pharmaceutical
Chemistry Department, College of Pharmacy, King Saud University, P.O.Box 2457, Riyadh11451, Saudi Arabia
2 Micro-Analytical
Laboratory, Applied Organic Chemistry Department, National Research Center, Doki, Cairo, Egypt
Accepted July 1, 2016
Published online September
7, 2016
The fabrication and development of two polyvinyl chloride (PVC) membrane
sensors for assaying phenobarbitone sodium are
described. Sensors
1 and 2 were fabricated utilizing b- or g-cyclodextrin as ionophore
in the presence of tridodecylmethylammonium chloride as a membrane additive, and PVC and dioctyl phthalate as plasticizer.
The analytical parameters of both sensors were
evaluated according to the IUPAC guidelines. The proposed sensors showed rapid,
stable anionic response (–59.1 and –62.0 mV per decade) over a relatively wide phenobarbitone concentration range (5.0 × 10–6–1×10–2
and 8 × 10–6–1 × 10–2 mol L–1) in the pH range
of 9–11. The limit of detection was 3.5 × 10–6 and 7.0 × 10–6
mol L–1 for sensors 1 and 2, respectively. The fabricated sensors
showed high selectivity for phenobarbitone over the
investigated foreign species. An average
recovery of 2.54 µg
mL–1 phenobarbitone sodium was 97.4 and 101.1 %, while the mean relative standard deviation was 3.0 and 2.1 %, for
sensors 1 and 2, respectively. The results acquired for determination of phenobarbitone in its dosage forms utilizing the proposed
sensors are in good agreement with those obtained by the British Pharmacopoeial method.
Keywords: phenobarbitone sodium, membrane
selective electrode, b-cyclodextrin, g-cyclodextrin, PVC, potentiometry