Acta Pharm. 66 (2016) 503-514


full paper

Original research paper


Ionophore-based potentiometric PVC membrane sensors for determination of phenobarbitone in its pharmaceutical formulations


1 Pharmaceutical Chemistry Department, College of Pharmacy, King Saud University, P.O.Box 2457, Riyadh11451, Saudi Arabia

2 Micro-Analytical Laboratory, Applied Organic Chemistry Department, National Research Center, Doki, Cairo, Egypt

Accepted July 1, 2016

Published online September 7, 2016


The fabrication and development of two polyvinyl chloride (PVC) membrane sensors for assaying phenobarbitone sodium are described. Sensors 1 and 2 were fabricated utilizing b- or g-cyclodextrin as ionophore in the presence of tridodecylmethylammonium chloride as a membrane additive, and PVC and dioctyl phthalate as plasticizer. The analytical parameters of both sensors were evaluated according to the IUPAC guidelines. The proposed sensors showed rapid, stable anionic response (59.1 and 62.0 mV per decade) over a relatively wide phenobarbitone concentration range (5.0 1061102 and 8 1061 102 mol L1) in the pH range of 911. The limit of detection was 3.5 106 and 7.0 106 mol L1 for sensors 1 and 2, respectively. The fabricated sensors showed high selectivity for phenobarbitone over the investigated foreign species. An average recovery of 2.54 g mL1 phenobarbitone sodium was 97.4 and 101.1 %, while the mean relative standard deviation was 3.0 and 2.1 %, for sensors 1 and 2, respectively. The results acquired for determination of phenobarbitone in its dosage forms utilizing the proposed sensors are in good agreement with those obtained by the British Pharmacopoeial method.


Keywords: phenobarbitone sodium, membrane selective electrode, b-cyclodextrin, g-cyclodextrin, PVC, potentiometry