Acta Pharm. 62 (2012) 547-562
Original research paper
Improved
photostability, reduced skin permeation and irritation of isotretinoin by solid
lipid nanoparticles
SHIVA GOLMOHAMMADZADEH, SOMAYE MORTEZANIA and MAHMOUD REZA JAAFARI
1 Nanotechnology
Research Center, School of Pharmacy, Mashhad University of Medical Sciences,
Mashhad, Iran
2 Biotechnology
Research Center, Nanotechnology Research Center, School of Pharmacy, Mashhad
University of Medical Sciences, Mashhad, Iran
Accepted August 27, 2012
The aim of this study was to develop new solid lipid nanoparticles
of isotretinoin (IT-SLNs) and evaluate the ability of IT-SLNs to improve
photostability, reduce skin permeation and irritating effects. IT-SLNs were
prepared by the hot high pressure homogenization method. Size, zeta potential and
morphological characteristics of the preparations were assessed by transmission
electron microscopy (TEM) and thermotropic properties with differential
scanning calorimetry (DSC). IT-SLNs had a small average
diameter of 74.05 ± 8.91 nm and high encapsulation efficiency (EE) of 80.6 ±
1.2 %. The results showed that the entrapment of IT into SLNs reduced
significantly its photodegradation. The in vitro permeation data showed
that IT-SLNs can accumulate in the different layers of the skin and prevent
systemic uptake of IT in mouse skin. IT-SLNs also significantly increased IT
accumulation in the different layers of the stratum corneum of human skin.
IT-SLN formulation was significantly less irritating compared to commercial
IT-GEL, which shows its potential for improving skin tolerability and being a
carrier for topical delivery of IT.
Keywords: SLN, isotretinoin, photostability, skin irritation, skin
permeation