Acta Pharm. 69 (2019) 75-86

Original research paper

Effects of annexin A1 on apoptosis and cell cycle arrest in human leukemic cell lines

AFFIDAH SABRAN, ENDANG KUMOLOSASI and IBRAHIM JANTAN

e_kumolosasi@yahoo.co.id; e_kumolosasi@ukm.edu.my

¹ Drug and Herbal Research Centre, Faculty of Pharmacy, Universiti Kebangsaan Malaysia, Kuala Lumpur, Malaysia

² School of Pharmacy, Taylor’s University, Lakeside Campus, 47500 Subang Jaya, Selangor, Malaysia

Accepted August 20, 2018

Published online September 16, 2018

Recent studies suggest that annexin A1 (ANXA1) promotes apoptosis in cancerous cells. This study aims to investigate the effects of ANXA1 on apoptosis and cell cycle arrest in K562, Jurkat and U937 cells and peripheral blood mononuclear cells (PBMC). Cells were treated with ANXA1 and cyclophosphamide prior to flow cytometry analysis for apoptosis and cell cycle arrest induction. At 2.5µM, ANXA1 induced significant apoptosis in K562 (p ≤ 0.001) and U937 (p ≤ 0.05) cells, with EC₅₀values of 3.6 and 3.8 µM, respectively. In Jurkat cells, induction was not significant (EC_50, 17.0 µM). No significant apoptosis induction was observed in PBMC. ANXA1 caused cycle arrest in the G0/G1 phase in K562 and U937 cells with p ≤ 0.001 for both, and (p ≤ 0.01) for Jurkat cells. ANXA1 induced apoptosis and cycle arrest in the G0/G1 phase in K562 and U937 cells, causing only cell cycle arrest in Jurkat cells.

Keywords: annexin A1, apoptosis, cell cycle, flow cytometry, human leukemic cell lines