Acta Pharm. 59 (2009) 89-96
Hepatoprotective effects of Calotropis gigantea extract against carbon tetrachloride induced liver injury in rats
GAURAV LODHI, HEMANT KUMAR SINGH, KAMLESH K. PANT and ZEASHAN HUSSAIN
1Department of Pharmacology and Therapeutics, Chatrapati Sahuji Maharaj Medical University, Lucknow, India-226003
2 Hygia Institute of Pharmaceutical Education and Research, Faizullahganj, Lucknow, India-226020
Accepted January 14, 2009
Ethanolic extract (50 %) of stems of Calotropis gigantea R. Br. (Asclepiadaceae) at doses of 250 and 500 mg kg-1 were studied for hepatoprotective activity in male Wistar rats with liver damage induced using carbon tetrachloride, 2 mL kg-1 twice a week. The protective effect of C. gigantea extract was compared with the standard drug silymarin. Various biochemical parameters such as aspartate amino transferase (AST), alanine amino transferase (ALT), glutathione (GSH), lipid peroxide (LPO), superoxidedismutase (SOD), glutathione peroxidase (GPx) and catalase (CAT) were evaluated. The results revealed that the C. gigantea extract significantly decreased AST, ALT (p < 0.001) and lipid peroxide (p < 0.01) levels. The antioxidant parameters GSH, GPx, SOD and catalase levels were increased considerably compared to the levels in groups treated with carbon tetrachloride only.
Keywords: Calotropis gigantea (Asclepiadaceae), hepatoprotective activity, serum enzymes, antioxidants