Acta Pharm. 57 (2007) 241-248

full paper

Short communication

 

Hepatoprotective effect of the ethyl acetate extract of Teucrium polium L. against carbon tetrachloride-induced hepatic injury in rats

 

TATJANA KADIFKOVA PANOVSKA1*, SVETLANA KULEVANOVA2, ICKO GJORGOSKI3, MIRJANA BOGDANOVA4 and GORDANA PETRUSHEVSKA5

taka@ff.ukim.edu.mk

1Institute of Toxicology, Faculty of Pharmacy, University “Ss. Cyril and Methodius”, 1000 Skopje, Republic of Macedonia

2Institute of Pharmacognosy, Faculty of Pharmacy, University “Ss. Cyril and Methodius”, 1000 Skopje, Republic of Macedonia

3Institute of Biology, Faculty of Natural Sciences and Mathematics, University “Ss. Cyril and Methodius”, 1000 Skopje, Republic of Macedonia

4Institute of Clinical Biochemistry, Clinical Center, 1000 Skopje, Republic of Macedonia

5Institute of Pathology, Faculty of Medicine, University “Ss. Cyril and Methodius”, 1000 Skopje, Republic of Macedonia

Accepted March 5, 2007

 

Hepatoprotective activity of the ethyl acetate extract of Teucrium polium (L.) was investigated using rats with CCl4-induced liver damage. Specific biochemical parameters (glutathione peroxidase, superoxide dismutase, reduced glutathione and total antioxidative status) were estimated in blood and in liver homogenate. Lipid peroxidation in CCl4-intoxicated rats was evidenced by a marked increment in the levels of thiobarbituric acid reactive substances. Histopatological examinations of the liver were undertaken to monitor the liver status. Silymarin was used as a standard to compare the hepatoprotective activity of the extract. Some biochemical parameters in groups treated with the Teucrium polium extract at a dose of 25 mg kg-1, showed significantly different values than that of the CCl4-treated group. The liver biopsy of all experimental rat groups treated with the Teucrium polium ethyl acetate extract showed significant restoration of the normal histomorphological pattern of liver cells. The study substantiates the potential hepatoprotective activity of the ethyl acetate extract of Teucrium polium L.

 

Keywords: Teucrium polium (Lamiaceae), hepatoprotective effect, carbon tetrachloride, sylimarin, glutathione